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egfp rubicon δ182  (Addgene inc)


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    Structured Review

    Addgene inc egfp rubicon δ182
    Egfp Rubicon δ182, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+rubicon+%CE%B4182/EGFP-Rubicon+(Plasmid+%2328022)/bio_rxiv__64898__2026__03__04__709622-216-9-11
    Average 92 stars, based on 9 article reviews
    egfp rubicon δ182 - by Bioz Stars, 2026-10
    92/100 stars

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    Transfection:

    Article Title: Rubicon modulates neuroimmune responses following traumatic brain injury
    Article Snippet: HEK293T cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco; 11995073) supplemented with 10% fetal bovine serum (FBS) (GeminiBio; 900-108H-500) and 1% antibiotic-antimycotic (Gibco; 15240062). .. HEK293T cells were transiently transfected with EGFP-Rubicon (Addgene; 28022), EGFP-Rubicon Δ182 (Addgene; 28041), EGFP-Rubicon ΔCT (Addgene; 28040), pcDNA3.1(+)-human LRRC33 (Addgene; 111602), and/or pEGFP-C1 vectors using Lipofectamine TM 3000 Transfection Reagent (ThermoFisher Scientific; L3000015) for 24 h.( , , ) The cell monolayers were washed with ice-cold PBS and lysed in cell lysis bucer (50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, and 1% Triton X-100 or 0.5% Igepal CA-630 (SigmaAldrich; I8896) containing protease and phosphatase inhibitors. .. The cell lysates were centrifuged at 18,000 xg for 30 min, and the resulting supernatants were pre-cleared with Pierce TM Protein A/G Agarose beads (ThermoFisher Scientific; 20421) and mouse mAb IgG1 (CST; 5415) or mouse IgG2a (CST; 61656) for 2 h at 4 °C with rotation.

    Lysis:

    Article Title: Rubicon modulates neuroimmune responses following traumatic brain injury
    Article Snippet: HEK293T cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco; 11995073) supplemented with 10% fetal bovine serum (FBS) (GeminiBio; 900-108H-500) and 1% antibiotic-antimycotic (Gibco; 15240062). .. HEK293T cells were transiently transfected with EGFP-Rubicon (Addgene; 28022), EGFP-Rubicon Δ182 (Addgene; 28041), EGFP-Rubicon ΔCT (Addgene; 28040), pcDNA3.1(+)-human LRRC33 (Addgene; 111602), and/or pEGFP-C1 vectors using Lipofectamine TM 3000 Transfection Reagent (ThermoFisher Scientific; L3000015) for 24 h.( , , ) The cell monolayers were washed with ice-cold PBS and lysed in cell lysis bucer (50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, and 1% Triton X-100 or 0.5% Igepal CA-630 (SigmaAldrich; I8896) containing protease and phosphatase inhibitors. .. The cell lysates were centrifuged at 18,000 xg for 30 min, and the resulting supernatants were pre-cleared with Pierce TM Protein A/G Agarose beads (ThermoFisher Scientific; 20421) and mouse mAb IgG1 (CST; 5415) or mouse IgG2a (CST; 61656) for 2 h at 4 °C with rotation.



    Similar Products

    egfp rubicon δ182  (Addgene inc)
    92
    Addgene inc egfp rubicon δ182
    Egfp Rubicon δ182, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+rubicon+%CE%B4182/EGFP-Rubicon+(Plasmid+%2328022)/bio_rxiv__64898__2026__03__04__709622-216-9-11
    Average 92 stars, based on 1 article reviews
    egfp rubicon δ182 - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

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